The Duke-NUS Metabolomics Lab is a leading metabolomics core and research facility in Singapore, actively contributing to numerous projects aimed at uncovering disease mechanisms through advanced metabolomics profiling.

We are seeking a Research Fellow to play a key role in metabolomics-related research. The successful candidate will work both independently and collaboratively to analyse biological samples using mass spectrometry and multi-omics approaches. The research focuses on understanding the molecular mechanisms underlying diseases such as Amyotrophic Lateral Sclerosis (ALS), metabolic disorders, and the role of the gut microbiome in these conditions. Independently carry out the design and execution of experimental research required by the research component of the project.

• Independently design, plan, and execute experimental research aligned with project objectives.

• Write and review research papers, present research outcomes, conceptualise new ideas and develop plans for independent research which could have a considerable influence and impact on the relevant field attracting research funding.

• Provide support for external grant/protocol development, study design and management of research activities.

• Source financial support for research activities by investigating potential sources of funding, collaborate with Principal Investigator to write and revise grant/fellowship applications.

• Provide guidance and mentorship to junior researchers.

• Ensure optimal progress of experiments in the laboratory by conducting, supervising, and evaluating laboratory procedures and outcomes. Revise and analyse targets to obtain high-quality and reproducible results.

• Perform other related duties incidental to the work described therein.

Working context: The Institute for Integrative Biology of the Cell (I2BC) is a large mixed research unit created on January 1, 2015. It is jointly supervised by the CNRS, CEA, and Université Paris-Saclay, with additional partnerships including INRAe, INSERM, and Université Paris Diderot, among others. Located 20 km from Paris, France, the institute fosters interdisciplinary research at the cutting edge of biological sciences (https://www.i2bc.paris-saclay.fr).

Host laboratory: Our research group (Protein Maturation, Cell fate and Therapeutics, https://www.i2bc.paris-saclay.fr/equipe-protein-maturation-cell-fate-therapeutics/), headed by C. Giglione, is seeking a highly motivated and skilled assistant engineer to join our team. Our group focuses on a range of modifications that affect proteins. These modifications share the common feature of targeting reactive amino groups, either at the N-terminus or on the side chains of lysines. The team aims to understand the nature and biological effects of these modifications as well as the impact of these modifications on the maintenance of a functional proteome. In this context, our research focus includes identifying the protein populations involved, exploring competition between different modifications on the same chemical group, and examining the physiological adjustment of their stoichiometry, which lies at the heart of proteostasis. Protein analysis and modification studies are conducted using mass spectrometry, leveraging instruments managed by the platform adjacent to the team’s location. The properties of the enzymes catalyzing these modifications are characterized both in vitro through biochemical approaches and in vivo through genetic methods (e.g., loss-of-function studies). The nature of the modifications can also be analyzed in cell extracts from various species using targeted approaches for specific amino groups or broader methods. In all cases, a quantitative analysis is prioritized and carefully examined. Computational tools and databases are developed to support these analyses. Additionally, biophysical in vitro studies or in-cellulo cellular biology approaches are employed to enhance the understanding of the distribution of modified proteins across different compartments.