Working context: The Institute for Integrative Biology of the Cell (I2BC) is a large mixed research unit created on January 1, 2015. It is jointly supervised by the CNRS, CEA, and Université Paris-Saclay, with additional partnerships including INRAe, INSERM, and Université Paris Diderot, among others. Located 20 km from Paris, France, the institute fosters interdisciplinary research at the cutting edge of biological sciences (https://www.i2bc.paris-saclay.fr).

Host laboratory: Our research group (Protein Maturation, Cell fate and Therapeutics, https://www.i2bc.paris-saclay.fr/equipe-protein-maturation-cell-fate-therapeutics/), headed by C. Giglione, is seeking a highly motivated and skilled assistant engineer to join our team. Our group focuses on a range of modifications that affect proteins. These modifications share the common feature of targeting reactive amino groups, either at the N-terminus or on the side chains of lysines. The team aims to understand the nature and biological effects of these modifications as well as the impact of these modifications on the maintenance of a functional proteome. In this context, our research focus includes identifying the protein populations involved, exploring competition between different modifications on the same chemical group, and examining the physiological adjustment of their stoichiometry, which lies at the heart of proteostasis. Protein analysis and modification studies are conducted using mass spectrometry, leveraging instruments managed by the platform adjacent to the team’s location. The properties of the enzymes catalyzing these modifications are characterized both in vitro through biochemical approaches and in vivo through genetic methods (e.g., loss-of-function studies). The nature of the modifications can also be analyzed in cell extracts from various species using targeted approaches for specific amino groups or broader methods. In all cases, a quantitative analysis is prioritized and carefully examined. Computational tools and databases are developed to support these analyses. Additionally, biophysical in vitro studies or in-cellulo cellular biology approaches are employed to enhance the understanding of the distribution of modified proteins across different compartments.

Join MSD on an exciting journey to support our mass spectrometry (MS) capabilities within the Target & Pathway Biology group (Singapore). We are seeking a highly motivated and detail-oriented Scientist to join our research team. The successful candidate will provide technical support and expertise in LC-MS and/or MALDI-MS Imaging-based OMICs technologies to support various pipeline projects in basic, translational, and clinical sciences across a diverse range of mass spectrometry (MS)-related applications in proteomics, lipidomics, and small molecule quantitation.

What will you do:
• With supervision be able to support, learn and operate mass spectrometry related activities including assay development, optimization, and mass spectrometer maintenance.
• Assist scientists with in vitro / in vivo / clinical tissue sample preparation for spatial data acquisition by MALDI-MS Imaging workflows and LC-MS based proteomics or metabolomics applications.
• With guidance from senior scientists, employ statistical, bioinformatics and data visualization tools to analyze and interpret LC-MS and MALDI-MS Imaging omics datasets.
• Contribute to and implement the Target and Pathway Biology group and the overall Quantitative Biosciences research strategy aligned with MRL’s drug discovery approaches and organizational objectives.
• Retain an up-to-date knowledge of the key therapeutic area biology, analytical techniques and equipment and carry out literature searches and provide reviews as required in support of priority projects with good communication skills.
• Work collaboratively on MSD research projects with matrix teams within the department and other cross functional teams at MRL to enable the scientific advancement of projects under scientific and administrative direction.
• Assist the group in inventory management, documentations for health and safety, biological materials, risk assessments, management of change documentation, international shipping and capital equipment purchases and maintenance.
• Ensure reliable documentation of experiments and results in electronic notebooks and other documents in compliance with company policies and project needs. Identify the need for and ensure the implementation of risk assessments and SOPs as appropriate.
• Proactively plan and execute own career development strategy with help of manager to advance technical and functional knowledge to design experiments/projects that contribute to overall direction of department/discipline.
• Demonstrate consistent leadership behaviors. Ensure all activities comply with appropriate health and safety legislation, company codes of practice and local rules. Promote a culture of safety and compliance among reports and colleagues.

RESPONSIBILITIES

• Design and application of appropriate analytical strategies (focusing on small molecules) to support development of high value healthy good ingredients with novel functionalities.

• Perform targeted and untargeted metabolomics experiments, LC-MS method development, method validation and troubleshooting, analysis, and interpretation of HRMS and NMR data for biomarker identification and validation.

• Improved productivity and streamlining of existing analysis methods (macro- and micro-nutrients) via automation or new methodologies.

• Supervision of postgraduate/undergraduate students and determine validity of data.

• Creative interpretation and presentation of data to provide insightful correlation.

• Writing reports, publications, conference papers, preparation of research proposals and budget planning. Participation in preparations of funding proposals will be expected.

• Interact and collaborate with a broad range of scientific disciplines with the existing research groups at SIFBI and A*STAR Research Institutes, particularly those focusing on integration of metabolomics, genomic, nutrition and bioinformatics.

• Contribute to the continuous improvement and future development of analytical capability.

Do you have hands-on experience with LC-MS instrumentation for proteomics and excellent people and communication skills? Then you might be the one we are looking for as our new Product Specialist based out of Singapore. We are experiencing increasing demand in the Asia-Pacific region, and we need you to assist our customers and service partners with service and application support as well as provide internal sales support.

Key Tasks and Responsibilities
• Support Evosep One users as a domain expert in LC-MS and proteomics.
• Plan and provide user training.
• Plan and provide installation, preventive maintenance and repair.
• Provide technical support from home-based office.
• Create support related documentation.
• Support our sales team with product expertise and scientific presentations.